FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005320338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005320338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30855794
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT19600.012.6118871
CGTATCG17250.010.5520943
GTATCGA17250.010.4218044
TCGTACG7350.09.20038629
TACCGTA21850.09.049849
CCGTATC26800.08.88822
CTCGGAT35200.08.4908741
CTACCGT23750.08.4206298
CGTATGG22250.08.38227712
ACCGTAT21600.08.21831910
CCGTATG22650.08.13501111
ATCGAAG38100.07.9031086
CACGAAT19900.07.79182961
TCGTATG27850.07.76959543
CTCGGTT24300.07.7681291
CCCGGTT24650.07.56666661
CGCGATT3308.918838E-47.49069641
CTCGCAT35400.07.4906961
ACTACCG27500.07.43578437
CTCGTTT30900.07.417971
Produced by FastQC (version 0.11.5)