FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005320339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005320339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26161426
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT20000.015.616531
GTATCGA17950.014.0199584
CGTATCG22000.013.1753153
CGTATGG21400.013.01897912
CCGTATG22350.012.16401411
ACCGTAT23400.012.09812110
CTACCGT27350.011.9119328
TACCGTA27150.011.6683889
CGACCGT8050.010.8860041
ACTACCG30500.010.4607037
CGTATCT21800.010.23867139
CTCGGTT20300.09.1871711
TCGTATG24050.09.06307345
ATCGAAG43000.08.9873656
CCGTATC38250.08.8703242
TCGTACG9500.08.77926329
CTCGTAT25450.08.56338744
GCGTAGC16450.08.35879929
TCGAAGG43150.08.2792067
CTCGGAT30400.08.2044441
Produced by FastQC (version 0.11.5)