FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005320341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005320341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29821670
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT17600.011.1070891
CGCGATT3807.089875E-89.4608471
CTCCGAT28000.09.1483011
CGTATCG13650.08.7242953
CGCGAAT3858.138919E-68.1707321
CTCGGAT36600.08.1651511
CGCAAAT20750.08.12151051
TCGTACG8950.08.05888629
CTCGTTT31950.08.0172911
GTCGAAT11150.07.85929161
CGGGGTT48050.07.85614631
CCCGGAT37950.07.8154821
CTCGACT28000.07.7038331
GTATCGA15450.07.56244044
CGACCGT7202.1464075E-107.48983761
CGCCAAT23000.07.42470841
CGGAGAT36000.07.3650071
CTCGAGT27300.07.24291941
CTCGCAT29550.07.2237011
CCGTATC22100.07.11693242
Produced by FastQC (version 0.11.5)