FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17482248
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA17900.010.6122289
CGACCGT10850.010.510291
CCCGTAT21700.010.510291
CGCGTAT3700.002364212610.2735721
GCATTCG27100.010.1644053
CGCGAAT4801.8234253E-49.8990141
CGATCTA7201.4688521E-79.89424
CATTCGG33150.09.5987184
CGCGGGT39300.09.30960751
CCGGCAT38950.09.14928151
CGGGGTT53100.08.9482621
CCGAGAT69950.08.7626451
GTATCGA17200.08.5596354
CGCGAGT20150.08.489081
CTCGGAT42700.08.457051
CGCCTAT18350.08.2860411
CGTATCG16050.08.2852613
CCGGTAT37950.08.2635251
CGGTAAT17350.08.2158971
ATCGAAG50900.08.2105676
Produced by FastQC (version 0.11.5)