FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17482248
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1039860.5948090886252158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT10950.011.7317831
TATCGCG4651.3158418E-410.2241479
CCCGTAT23350.010.18821
GCATTCG26550.09.6688373
CGCCAAT26450.09.5337681
CGACCTA8551.8135324E-89.4518714
GTATCGA16250.09.3612014
CGCAAAT19950.09.3011381
CCCGGTT33450.09.1032871
CATTCGG33650.09.0412794
CGTATCG18150.08.6433533
CGGCAAT24850.08.6158971
TGCGCGA18350.08.5498389
CCGAGAT66450.08.4489231
CTCGTAT16450.08.387771
CCGGTAT39450.08.3217841
CGAGAAT32400.08.2235131
CGCAGTT43300.08.1312671
CGGGAAT38250.07.96091271
CGCCACT70050.07.94679641
Produced by FastQC (version 0.11.5)