FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16273553
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA13800.011.0187094
CCGGGAT49350.010.8783161
CGTATCG14900.010.8425083
GTATCGA20050.010.1909314
CGCTAAT8401.3720637E-89.6147941
CGCGTAT4650.00150139419.1951731
GTCGATT14000.09.16233351
CCGGAAT35800.08.8912361
CCCGTAT23050.08.8627381
CGGGAAT39600.08.757861
CGGGGAT46050.08.562861
CGCCAAT33200.08.4427571
TACGCAC15800.08.4192619
GCGAACG10759.687938E-98.3981243
CGGGGTT52950.08.1648041
CGCGGAT20900.07.95595171
CCGGCAT44800.07.84736871
CTCGGAT51300.07.7791531
CGCGAAT8551.5688443E-57.7791521
CGGGCAT57950.07.706281
Produced by FastQC (version 0.11.5)