FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15707663
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT9800.011.6343841
CGCGTAT3450.001322152111.0161321
CGACCGT9457.2759576E-1210.5571261
CGCCAAT31000.010.4209051
CGCGGGT22800.010.0014881
CCGGAAT32650.09.8942751
CCGGGAT47100.09.78383351
GTATCGA18750.09.62966254
CCCGGTT28250.09.2491651
CGTATCG13900.08.8873753
CGCAAAT20350.08.87109851
CGCGAAT5901.4690842E-48.857251
TACGCAC14050.08.7914849
CCCGGAT55700.08.784971
GTCCGAT13751.8189894E-128.637651
GCATTCG25000.08.5523893
CGACCTA11756.148184E-108.4920044
CCGAGAT67800.08.47841
CTCGGTT27900.08.3435351
CGGGGAT41400.08.1473471
Produced by FastQC (version 0.11.5)