FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15463658
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT23750.015.9999041
CGTATCG14150.011.748023
GTATCGA19250.011.5962464
CCCGTAT23250.010.8278931
TACCGTA11900.010.3771519
CGACCTA10851.8189894E-1210.068124
CGAGAAT32050.09.7815321
CCGGGAT43900.09.3051841
CGCCAAT27600.09.2934231
GCATTCG24350.09.1675223
CGCCAAC36800.09.1643481
GCGGGTC39050.09.0009362
CGCCATT43800.08.8926411
CGCGTTT10203.803507E-98.8479861
CTCGGAT45650.08.8444161
CGGTAAT15650.08.8018651
CGGGGAT46000.08.7771211
CGCGCAT7754.2146694E-68.5805941
CCGTATC25150.08.4986812
CGGGTCT58700.08.2530633
Produced by FastQC (version 0.11.5)