FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12328398
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT17050.013.9317921
CGTATCG9250.012.3277473
CGACCTA10350.011.0178654
TACGCAC10801.8189894E-1210.1170329
CCCGGAT40500.09.7360861
CCCGTAT17150.09.695391
CCGGGAT29950.09.5173441
CGCGAGT9701.5334081E-99.3055751
GTATCGA13401.8189894E-128.8646544
CGCGGAT14501.8189894E-128.518571
CCGGCAT36600.08.4371091
CTCGGAT36000.08.3137981
CCCGCAT29350.08.0932551
CGGGAAT28600.07.9732721
CTCGTAT15152.3646862E-117.83950661
CGCCTAT12853.5470293E-97.7638571
CTCGGGT36750.07.75631241
CGCCAAT24700.07.6935071
CAATCGA13651.4133548E-97.65802154
CGGGTCT47950.07.6298563
Produced by FastQC (version 0.11.5)