FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14523158
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA19600.011.8731814
CGCGTAT3752.069689E-411.40378951
CGTATCG15200.011.2483153
CGCGGGT22450.010.370931
CGACCTA9205.4569682E-1110.3245064
CCCGTAT22000.09.5031591
CTCGGAT40500.09.1511891
CCGGGAT40350.08.2431361
CCGGCAT37550.08.2251031
CGCGAAA5501.470471E-88.203938542-43
CGCAAAT21250.08.0497341
CGGAAAT35450.08.0421651
CGAAAAC23250.07.9703911
CCGGAAT31850.07.9068661
CGCATAT9154.59737E-67.7894741
CGCCATT40500.07.7433141
CGCGAAT6150.002150777.72614531
CGGGGAT37600.07.70867871
CGCGGAT16653.6379788E-127.70526361
CGCACAT25900.07.70526361
Produced by FastQC (version 0.11.5)