FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18233828
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1095420.6007624948529733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA16250.012.2860014
CGCGTAT4405.709144E-611.8950861
CGCGGAT21300.09.8288041
CCGGGAT50850.09.731281
GTATCGA23450.09.7300044
CGTATCG17100.09.72963
CCGCAAT28650.09.6323211
CCCGTAT26550.09.4981491
CGACCGT11252.5465852E-119.3046011
CGCCAAT34600.08.9385051
CCGGGTT48950.08.4565691
GCGAACG12955.4569682E-118.4426953
CGCCAAC46000.08.3782791
CGAGAAT40550.08.330961
TACGCAC20750.08.2483349
CGGGAAT46550.08.0748611
ATTAGCG8903.0863503E-68.0108086
CGCGAAT10102.637862E-78.0085741
GTCGGTT27950.08.0009891
CGAAGAT70650.07.8795481
Produced by FastQC (version 0.11.5)