FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12284926
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT2953.5475392E-412.8841211
CGCGGGT17800.012.54482651
CGGGGAT32550.010.5091671
CGCGGAT15200.09.6895791
GTCGAAT12051.10958354E-108.674061
CTCGGAT36650.08.5557231
CGGGTCT48000.08.41144853
CGGACAT30600.08.3841521
CCGGCAT33450.08.3799741
CGGGGTT39900.08.3351221
CCGGGAT34200.08.1962031
CGACCTA10203.1735544E-77.9164324
CGAGAAT26600.07.85882851
CGGAAAT29700.07.83838221
CCCGTTT24900.07.8229641
CGCGTCT15252.7284841E-117.78855661
CGCGAAT8601.6945594E-57.73421761
CCGGGTT39600.07.67841531
CGGGCAT45900.07.6595961
CGCCATT33100.07.60737281
Produced by FastQC (version 0.11.5)