FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15429680
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT2852.6539504E-413.3341291
CGCGGGT33750.011.1191821
GCATTCG24950.011.0414193
TGCGCGA15200.010.6239819
CGACCGT9054.0017767E-1110.4978651
CGCGTTT9601.2187229E-109.8964241
CGCGGAT16850.09.8670581
CGACCTA8451.5181286E-89.5553894
CGCGAAT4600.00136184359.2940331
CATTCGG30600.09.1577084
CGTCGAT12301.8189894E-118.8826451
CGGCATA14600.08.7847711
CCGGTAT32800.08.6895431
CGACAAT19300.08.6145041
CGCGAGT17700.08.5880831
CGGCAAT21400.08.4350821
CTCGAAT31750.08.3784531
CCCGGAT54600.08.17814351
TATCGCG4402.655499E-68.09578920-21
CCCGTAT19950.08.0957211
Produced by FastQC (version 0.11.5)