FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15429680
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT2657.92297E-616.137741
CGCGATT5503.8045982E-812.0951541
GCATTCG23550.011.9057033
CCCGTAT16900.011.8088781
CGCGGGT34550.010.7273541
CGTATCG16500.010.3684183
CGCGAAT5052.670475E-510.3501671
TGCGCGA17550.010.2861099
CCCGGAT54800.09.8848551
CATTCGG29050.09.4880244
CTCGTAT16100.09.4443081
GTATCGA15600.09.4434584
CGACCGT7151.4087309E-69.3039641
CGCGAGT18500.09.2464881
CGCCATT35350.09.1404071
CGACCTA8501.67689E-78.9453014
CCGGTAT36000.08.8433811
ACGTGCG33150.08.7410066
CGACAAT18200.08.6156611
TAACACG29200.08.1372882
Produced by FastQC (version 0.11.5)