FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12166683
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT15800.012.6279341
CGACCTA9651.8189894E-1210.8280384
CGCGAAT9457.2759576E-1210.5566861
CGCCAAT22950.010.1426971
CGACCGT9256.493792E-109.7578021
CGGGGAT32750.08.9933291
CGCGCAT7001.0852786E-58.8223721
CCGGGAT33700.08.5988441
CTCGGAT36850.08.5083731
CGGGTAT10851.1472366E-88.3188631
CGCAGTT26400.08.2774021
CGCCGAT19200.08.1649371
GTTCGAT13701.8189894E-107.9753061
CGGGTCT47900.07.93277173
CGAAGAT46800.07.91751531
CGGGCAT46400.07.8833871
CCCGGAT43000.07.7333861
CGGGGTT42850.07.64959341
CGGCAAC21750.07.6444971
TCGCGCG6857.356862E-47.62701756
Produced by FastQC (version 0.11.5)