FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15454732
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAAT33850.011.3711
CGTATCG12950.010.6424423
CGCGAAT6001.5185105E-610.2959011
CGCGGGT24950.010.0943341
CGCGCAT8301.12377165E-89.732921
CGACCTA13250.09.6842634
CCCGTAT20800.09.5952931
CGCGAAA6504.223666E-69.5039091
ATTCGCG5103.3633478E-49.3140916
CTCGGAT44400.09.3112631
CGGGGAT45300.08.4968731
CGCGGAT16350.08.4285441
GTATCGA20450.08.1337994
CTCGTAT14801.4551915E-118.0269511
CGCTATT13106.1118044E-107.98038241
CGCGTAG8351.140339E-57.9673491
CCGGGAT44100.07.86604831
CCGGCAT36450.07.82214831
CGCCTAT14002.8740033E-107.80678271
CGCCATT42700.07.79008961
Produced by FastQC (version 0.11.5)