FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16631343
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA3956.510163E-915.63966751
CGACCTA8800.014.040244
CCCGTAT23650.011.6540831
CGCGTAT3750.002640400310.1377131
CCCGGTT32150.09.6075721
CTCGGAT52000.09.4127211
CGCCTAT14300.09.3047191
GTATCGA19550.09.2367824
CGCGAAT5304.922659E-48.9661381
CCGGTAT34350.08.8538981
CGCGGAT20850.08.6608161
CGCTAGT6908.754296E-58.264441
ACCGGTA13700.08.14644244-45
CCCGATT22750.08.1463771
CCGGGAT49200.08.1132611
CGTATCG20000.08.0793653
CGCGAAC8351.1400449E-57.9675141
CCGGAAT44400.07.92008831
CGCCAAC44600.07.88457251
CGTCGAT13952.6557245E-107.8349251
Produced by FastQC (version 0.11.5)