FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11427698
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG3350.001034865211.3436757
CCCGTAT17600.09.9877581
CGACCTA8903.441528E-99.6098494
CGTATCG9701.5297701E-99.30676653
CTCCGAT26400.09.1779381
CGGGGAT27250.09.0660011
CGACCGT5801.2192221E-49.010391
CCGGCAT31150.08.9985571
CGCCAAT20600.08.9944861
CGAAGAT44650.08.9379251
GTCGATT10304.5147317E-98.7638581
CGCGGTT14100.08.7605861
CGCAAAT15300.08.6945181
CGGCAAT19750.08.6599281
CTCGGGT31600.08.5697211
CTCGTTT22150.08.3650741
AGTCGCG6858.075153E-58.3217836
CGGCGAA12954.8567017E-108.0710831
CTCGCAT25000.07.9815681
CCGGGAT31750.07.93069171
Produced by FastQC (version 0.11.5)