FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15355429
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA11300.015.5518574
CCCGTAT27250.013.4238341
CGTATCG19850.012.6822273
GTATCGA23750.011.7990584
CCGTATC30600.010.0895572
CGACCGT9902.1827873E-109.5972571
TACGCAC16900.09.5552649
CCGGGAT41300.09.317241
CTCGCGT13351.8189894E-128.8963341
CGCGAGT11554.4019544E-108.6375321
CGCGGTT14701.8189894E-128.4024961
CGCTAAT7452.3773817E-58.2897111
CCCGGAT50600.08.0742141
ATCGAAG46650.08.0433816
GTCCGAT11252.1842425E-88.0233061
TATCGAA38150.07.9679365
CGCGCAT9003.6449637E-67.9177371
CGCTAGG9651.16101E-67.87671231
CGGGCAT58300.07.7411831
TAATTCG12600.07.728137514-15
Produced by FastQC (version 0.11.5)