FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22333971
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG21400.010.8745763
GATCGGA119100.010.22915394-95
CGCGAAT12750.010.0622171
CCCGTAT33400.09.8161851
GTATCGA29550.09.8039794
CGCGTAT4902.2439557E-49.6971481
CGCGTTT16700.09.6739221
AGATCGG152100.09.32135894-95
CGACCTA17750.09.09725954
CGCGGAT28900.09.0428431
CGGGGAT60850.08.8238481
CCGGAAT49900.08.5700251
CTCGGAT68450.08.468891
CGACCGT13006.002665E-118.4066821
CTCGGTT38450.08.4033551
CGCTAGT9054.37607E-78.4006231
CCCGGTT43500.08.1924181
CCCGGAT80750.08.1792291
CCGGGAT59400.08.0793251
CGGGGTT71200.08.0750541
Produced by FastQC (version 0.11.5)