FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19833158
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT6604.0854502E-810.7979371
CGTATCG18550.09.730123
CCCGTAT32550.09.3416251
GATCGGA110900.09.22936494-95
CGACCTA14950.09.2134484
GTATCGA26900.09.0049914
CCGGGAT53500.08.9693531
CGGGGAT50350.08.8699641
CGCCATT57550.08.8334821
CGGAAAT55350.08.7554011
CTCGGAT59200.08.6675331
CGCCAAC47400.08.5198921
AGATCGG141700.08.49697194-95
CGACCGT13006.002665E-118.4057791
CGCGTCT21000.08.3709731
AGTCGCG12753.5470293E-108.1959596
CTCGGTT34800.08.1915391
CTCGGGT59000.08.1332261
ATCGGAA108300.08.06947894-95
TCGCGCG10657.40165E-88.0280518
Produced by FastQC (version 0.11.5)