FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16451050
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG6150.010.42580420-21
CGAAGAT60500.010.1289811
CGACCGT10455.456968E-1210.000871
CGACCTA10002.5465852E-119.9740794
CGCGGAT19100.09.948511
CGCGAAT6152.2778442E-59.2690991
CCGGGAT50100.09.1025891
CTCGGAT49050.08.9100511
CGCCAAT34250.08.5992881
CCCGTAT24400.08.3716311
GTATCGA18750.08.3592284
GTCCGAT13157.6397555E-118.3087081
CCGAAAT29800.08.2893111
CCCGGAT63100.08.2812281
CGCGAAA8950.08.229842-43
CCGACAT41100.08.206311
CGGTAAT15700.08.16951
CGTATCG20050.08.0546333
CGCGATT5350.0053478467.9913491
CGCCAAC44600.07.98836331
Produced by FastQC (version 0.11.5)