FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14116519
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG11700.012.5845643
CGCGGGT25300.011.82821751
CCCGTAT19000.011.2501471
CGACCTA10250.010.6575794
CGACCGT8208.385541E-1010.4269651
CGCGATT4852.0303407E-49.79394151
GTATCGA17600.09.7150374
CGCGCAT7854.9654773E-89.6816551
CTCGGTT22100.09.6720711
TATCGCG5305.8686237E-78.961279
CGGGTCT55150.08.9567443
CGCCAAT26050.08.9348561
CGCGGAT16100.08.8510471
TACGCAC11959.458745E-118.7437999
CCGCAAT21250.08.4942281
CTCGAAT31850.08.3517571
CTCGGAT40500.08.3272691
GCATTCG22850.08.31453
CGCCATT36300.08.2439091
GTCGATT10052.502493E-78.034931
Produced by FastQC (version 0.11.5)