FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16433073
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT51300.010.3713621
CGCCAAT34050.09.90549851
CCCGTAT21850.09.7835441
CGACCTA9307.1486284E-109.705734
GTATCGA18200.09.6580164
CCGGTAT36050.09.4877311
GTCGATT14600.09.1104591
CGAAGAT59700.08.9120751
CTCGGTT29450.08.2265921
CGCCGAC26550.08.0516171
CGCGTCT18300.08.0472171
CGGTATT37800.08.043392
CGCGGAT19650.07.97786241
CGCGTTT12602.4228939E-97.91742321
GCATTCG23600.07.85063743
CCGCAAT25000.07.7907451
CGCGGGT29900.07.62614731
CGCCAAC42400.07.6186531
CGGGAAT37800.07.54040341
CCGGCAT42950.07.52109151
Produced by FastQC (version 0.11.5)