FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11282725
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG11250.013.5109593
CGCGAAT5453.3607648E-812.203321
CGCGTAT3050.006642701610.9029661
CGCGGAT14550.09.7950071
CCGCAAT16600.09.73011
CCCGTAT15650.09.7136421
CCGGGAT29600.09.629551
GTATCGA14500.09.4993034
CCCGGAT38800.09.4276931
CCGTATC16700.08.8174612
CTCGGGT31550.08.7332341
CCGGAAT21900.08.6768551
TACCGTA9408.558527E-88.5895679
CCGAAAT20050.08.52971651
CCGATAG8057.012075E-68.2618751
CGGGAAC30550.08.086091
GAATCGG16500.08.0600154
CGGAAAT28250.07.90361641
CTCGAAT25400.07.6682561
CGCCCTT24800.07.66222331
Produced by FastQC (version 0.11.5)