FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11282725
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT2002.7549113E-416.6314221
CCGGGAT31400.011.6525891
TATCGCG2900.00456283611.4668959
CCCGGAT38850.09.9073011
GTCGATT10901.4551915E-119.5908591
GTCCGAT11807.2759576E-129.2620521
CGTATCG9501.0515578E-89.0044753
GTATCGA12352.0008883E-118.8505924
CGACCTA7552.9407038E-68.8123664
CCCGGTT21500.08.6196071
CGGGGAT29600.08.1872831
GATCGAA14609.094947E-128.1376435
CGGGAAT21700.08.1022071
CGCCAAC28700.07.947321
CCCGTAT16750.07.94336561
CGCAAAT15052.0008883E-117.89341351
CGGCGAA11452.9718649E-87.8851421
CCGGAAT22350.07.8665721
CACGCGT6655.345603E-47.85808759
CTCGTTT24950.07.6181721
Produced by FastQC (version 0.11.5)