FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15293233
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA10300.013.83402254
GTATCGA22350.012.7508224
CCCGTAT25800.011.9694211
CGTATCG18050.011.57873
CCGTATC29900.09.6904292
CGCCATT39650.09.2262881
TATCGCG4657.381459E-99.192615522-23
CGCGGAT17700.09.1260991
CGCACAT27300.08.7013541
CGGGGTT54650.08.6933941
CCCGGAT53650.08.5897691
TACCGTA16750.08.2236249
CGGGATT25550.08.1816571
CCGCAAT22650.08.1804261
CCGGGAT43100.08.1570641
CGCGAAT5900.00143113918.0524391
CGACCGT8401.2385421E-57.91823241
CGCGCAT8401.2385421E-57.91823241
CCGGTTT25200.07.91823241
CTCGGTT23600.07.85112761
Produced by FastQC (version 0.11.5)