FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11399284
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA6301.1641532E-1012.8222614
CCCGTAT17650.012.6531851
CTCGGAT33550.010.0557041
CCCGGAT38900.09.4056281
GCGAACG7703.86206E-79.2573623
CGTATCG11453.8198777E-119.1306963
TCGGATT16700.09.10584452
CCGGGAT27900.09.0264791
CGCCATT28000.08.9942411
CCCGGTT22050.08.4043231
CTCGAAT23900.08.3502241
CGGGGTT38450.08.0327411
CGCGACT7151.3257856E-47.97483871
CCGGAAT21500.07.9562921
GATCGGA44650.07.930658394-95
GTCGGTT15202.5465852E-117.81525471
CGCGGGT15252.7284841E-117.78963041
CTCGGTT20850.07.7485341
CTCGGGT31500.07.69318771
CCCGAAC24300.07.62614441
Produced by FastQC (version 0.11.5)