FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12751199
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA2600.00201559612.7886431
CGACCTA9600.011.3790314
CGCGCAT6654.5783963E-810.71443751
CGACCGT9054.0017767E-1110.4973861
CCCGTAT17700.010.466251
CGCGGTT13950.09.8746921
CTCGGGT37200.08.8106081
CCGGCAT32600.08.5967471
CGCGCGT6152.3067575E-48.4960561
CCCGGTT24700.08.46165851
CCCGTTT26950.08.4602321
CGTATCG13006.002665E-118.4030093
CGCGGGT17950.08.2034591
CGGGTAT12854.1654857E-108.1324111
CCGTAAT12351.6425474E-98.0770381
CGCGAAA5451.081753E-77.84941242-43
CGCATAT9104.2721877E-67.82978151
CGCGTCT14659.276846E-117.78168061
CCGGAAT30600.07.6063171
TACCGTA11251.8639912E-77.5991249
Produced by FastQC (version 0.11.5)