FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12751199
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT3105.391597E-412.2600361
CGACCGT8205.456968E-1211.587231
CGCGGGT19050.010.9728931
CGTATCG11050.010.7487993
CGCGCAT8006.6344E-89.5015291
CGCCATT35700.09.3152251
CGACCTA8903.515197E-89.0750274
GTATCGA15850.08.9925124
CTCGTTT26000.08.7706421
CCCGTAT19300.08.61537551
CCGTAAT11857.2941475E-108.4190761
CGGGAAT29500.08.37422851
GTTCGAT14255.456968E-128.3346741
CGAGAAT28650.08.2910381
CGCCTAT13802.5465852E-118.2621991
CGGTAAT13309.640644E-118.2156081
CCGCAAT19100.08.2081271
CGCTAAT7051.12811875E-48.0864081
CCCGGAT42450.08.0578341
CTCGGAT37150.08.0564781
Produced by FastQC (version 0.11.5)