FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15125394
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA9800.018.4169944
CGTATCG17800.016.0107823
GTATCGA22650.014.8885144
CCCGTAT26100.014.1968311
CCGTATC27250.013.4218162
GTCCGAT11650.011.4174591
TATCGAA34200.010.1379325
CTCGGTT23100.09.8711241
ATCGAAG42650.09.577126
TACGCAC15400.09.5608499
TATCGCG4751.0568328E-99.49910420-21
CGCGAAT5801.2205629E-49.0095271
CGGGATT23500.08.8945121
CGCGGTT14400.08.5772521
CCGGCAT43600.08.3896061
CCGCAAT22800.08.3341711
CGGGGTT51550.08.2937551
TACCGTA14457.2759576E-128.2172719
CCGGAAT29850.08.1163941
CCCGGAT52500.08.0531911
Produced by FastQC (version 0.11.5)