FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13580132
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT2359.40531E-414.1490671
CGACCGT8200.012.1647461
CGACCTA9407.2759576E-1210.6107854
CCCGTAT16550.09.7583981
CCGGGAT46950.09.6113771
GTATCGA13950.09.5332254
CGCCAAT22150.09.2213041
TACGCAC12001.0913936E-119.10331259
TGCGCGA17750.09.0977379
CCGGTCT26750.09.0561581
TCGCGCG6959.937345E-68.8841248
CGCGGAT15050.08.8372911
CGTATCG14100.08.7582693
CGCCATT30000.08.7084141
CTCGGTT23600.08.4534671
CTCGTTT28700.08.4408441
CCGGAAT28750.08.2609461
CCCGGTT25100.08.1375251
GCATTCG21050.08.1229633
CGGTAAT11252.1889718E-88.0222961
Produced by FastQC (version 0.11.5)