FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14277062
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT24800.013.4126121
TATCGCG4706.4357664E-813.13999759
CGCCAAT27350.011.4670991
CCCGTAT19400.09.7977251
CGGGTCT55050.09.4961263
GCATTCG23550.09.484583
CGCGCAT8209.668656E-89.2719931
CGACCTA10851.2732926E-109.1965434
TACGCAC12805.456968E-128.9074039
CTCGGGT37050.08.5931741
CGCCATT36800.08.5224241
CCCGGTT28100.08.4553321
CTCGCGT10708.902134E-98.4379481
CGCGGTT14103.6379788E-128.4253491
CGTATCG10759.657015E-98.3995543
CGGGGAT39300.08.3430241
GCGGGTC40150.08.2855942
CCGGGAT38900.08.1845011
CGACCGT8852.858109E-68.0540621
CTCGTAT14951.8189894E-117.94631531
Produced by FastQC (version 0.11.5)