FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17275075
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG18450.011.5885243
CCCGTAT25450.011.5736581
CGCGGAT18700.010.6702451
CCGGTAT23500.09.703761
GTATCGA23750.09.4029164
CGACCGT11252.7284841E-119.2904531
CCGCAAT26150.09.2654211
CCGGGAT50200.08.7066441
CCCGGAT60500.08.6378171
CGCGTTT13301.0913936E-118.5728721
CGCCAAT37800.08.1693641
CTCGTAT19000.08.0013471
CGACCTA10102.6967064E-77.99752954
CCCGAAT54850.07.9685241
CGCCATT45000.07.91799931
CTCGGAT51300.07.8716951
CGCTAAT8451.3412249E-57.87114761
CGCGGGT27850.07.8469221
ATCGAAG48600.07.81929546
CGGCAAT30150.07.7210341
Produced by FastQC (version 0.11.5)