FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15456702
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA10050.015.121984
GTCGATT12250.09.6972651
TACGCAC13750.09.6704739
CGACCGT9155.5406417E-99.3475281
CGCCAAT26350.09.0164321
GTATCGA15450.08.9144434
CGGGAAT34450.08.8274671
CGTCGAT11353.110472E-108.7916181
CCGGGAT50250.08.6995561
GCATTCG24000.08.5090673
CGCGAAT8953.6940583E-78.4945881
CACGAAT19500.08.28494551
ATCGAAG44800.08.1624246
CCCGTAT20050.08.0576781
CGAAGAT55100.08.0200431
CGCGAAC8301.0532276E-58.0148481
TCCGATC16051.8189894E-127.99062732
ATTAGCG5950.00156152687.98159176
CCCGGAT58050.07.93989751
CGGAAAT36100.07.89749571
Produced by FastQC (version 0.11.5)