FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16032765
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA13850.015.4409744
CCGGGAT43100.011.9072011
TACGCAC16950.011.7712399
CGTATCG15600.010.6623123
CGCGGAT20000.010.4540821
GCGAACG10505.456968E-129.95729353
CGGCGAA18800.09.8575731
CCCGTAT24250.09.797641
CGACCGT9256.4574124E-109.7605681
CTCGGAT49950.09.0375631
CTACGCA27000.08.6208058
CCCGGTT29950.08.5676191
CCCGGAT60000.08.553341
GTATCGA19550.08.5081154
CGGGCAT58950.08.4638641
CGCCAAT31550.08.4343551
CGCCAAC40600.08.4269351
CGCTGAT34550.08.2521361
CGCGCAT9356.242086E-67.6232971
GTTCGAT19400.07.5931711
Produced by FastQC (version 0.11.5)