FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15112045
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG17850.014.9008383
CCCGTAT26800.014.8874791
GTATCGA22550.014.1119734
CGACCTA9700.013.220614
CCGTATC28800.011.8745372
TATCGCG4759.094947E-1210.49879320-21
CGCGGAT18000.010.0273871
CTCGGTT22450.09.0976411
CTCGTTT26350.09.0129321
ATCGAAG41700.08.8842526
CGACCGT9651.3875251E-88.8597581
CGCTAGT6051.9328781E-48.6360271
CGGGGAT43150.08.4759161
CCGGCAT39050.08.3927581
CGCGCAT8652.075214E-68.2366731
TATCGAA35600.08.138275
CCGGGAT41500.08.0117361
CTCGAAT31650.07.9538761
CGCGGGT22300.07.88085841
CGGCGAA17501.8189894E-127.5997041
Produced by FastQC (version 0.11.5)