FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14325172
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG12050.011.8288263
CGACCTA11150.011.5056584
CGCGGGT24600.011.393791
CGACCGT8501.0913936E-1111.1779561
CGCGTAT3852.6469538E-411.1053711
GTATCGA15350.010.5242984
CCCGTAT21300.010.4826131
CGCCAAT27000.09.5012621
ACGTACG5103.3619188E-49.3144756
CCGGGAT38950.09.1475561
CTCGTTT27700.08.9181521
CGCCATT37500.08.8678461
TACGCAC12905.0931703E-118.4702659
CCGCAAT21100.08.1053421
CCCGTTT31650.07.95524361
GTCCGAT12552.240995E-97.94926361
CCCGGAT49050.07.9419321
CTCGTAT13802.1282176E-107.91771841
GCATTCG22950.07.86698533
CCGGCAT33950.07.8360931
Produced by FastQC (version 0.11.5)