FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13601628
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT17900.010.6196341
CCGGGAT45400.010.1535631
CTCGCAT33150.09.8916371
CGCGTAT4000.00450134989.5045721
TCGCGCG5557.552933E-59.4148938
CGCCAAT25250.09.2222581
CGTATCG14500.09.1779083
CCGGCAT32550.09.0519731
CGACCTA9105.008951E-88.8790084
CGCGTAG8602.0050356E-78.8414631
CGCGAAT6453.9728453E-58.8414621
CGGGAAT31500.08.7502411
CCGTTTA20500.08.578374
CGCAGTT31750.08.5316631
CCGGTTT23350.08.3444851
CCGGTAT34400.08.2888721
TCGCATT26450.08.2657882
CGGCAAT22100.08.1713511
ATCGAAG40700.08.1697396
TGCGCGA15750.08.14399
Produced by FastQC (version 0.11.5)