FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19888453
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA16200.012.6121334
CGTATCG19600.011.3935013
GTATCGA25700.010.3535664
CGCGTAT4155.2935217E-410.3029021
CCCGTAT32100.09.4719661
TACGCAC18550.09.2198019
CGACCGT11504.1836756E-119.0884531
CTCGGGT64300.09.08781051
CGGTAAT19100.08.9543551
CCGGGAT52600.08.8512681
CCCGATT30650.08.8350611
GATCGGA110350.08.8287794-95
CGCCAAC46900.08.610161
CGGGGAT52700.08.564031
AGATCGG140200.08.49138394-95
CGCCATT53700.08.4045511
CCCGCAT49650.08.3246351
CGCGAAT12009.331416E-108.3138691
CGCGGGT26400.08.2778791
CCGCAAT31150.08.2357061
Produced by FastQC (version 0.11.5)