FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005321266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005321266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19571463
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG18950.013.2868973
CCCGTAT30950.010.744371
CGACCTA18750.09.8815414
GTATCGA26700.09.7861714
CCGCAAT31800.09.7102371
CGGGGAT51150.09.5661051
CTCGGAT59150.09.3966861
GTCGATT17350.09.3094281
CCGGGAT52250.09.0919551
CGCCAAT36450.08.8624741
GATCGGA106950.08.79879994-95
CGCGAAT12703.6379788E-118.6033531
CTCGGGT62300.08.4640561
CGCGGAT23200.08.3953621
CGACCGT11453.4087861E-98.2978981
CTCGTAT23650.08.235621
TCGGATT30750.08.1881862
TACGCAC17450.08.16721259
AGATCGG137650.08.16570994-95
CGAAGAT73650.08.1272081
Produced by FastQC (version 0.11.5)