FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4505643
Sequences flagged as poor quality0
Sequence length19-137
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT258540.5738137708646691No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG62610.13895907864870785No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC59780.13267806614949298No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA48860.10844179177089708No Hit
CTGCATCAAAAGAAAGGTTCAACTCTGTTAGTTGAGGACACACATCACAA47830.10615576955386834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT96650.0101.296823
CTGCATA128400.0100.713191
CATAGTA91900.099.460894
TGCATAG104650.097.406762
ATAGTAT94250.094.49315
GCTATAA65800.091.6333
CTGCTAT104300.091.4449841
TGCTATA69500.089.786052
GTATTCC99950.089.7675558
AGTATTC101000.089.190837
TAGTATT104100.086.88266
CAGCTGC49600.084.6815261
CTGCTGC55500.084.3531951
CTGCAGC55500.079.257311
CTGCAGT160250.078.2178961
TATTCCA116400.078.167859
CAGCAGC62400.077.62941
AGCACTA50900.076.844092
CTGCGTA11000.069.474591
CTATAAA73150.069.0172654
Produced by FastQC (version 0.11.5)