FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4505643
Sequences flagged as poor quality0
Sequence length19-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT47510.10544554905925747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT79400.093.788295
GTATTCC82500.090.9501348
AGTATTC85900.089.336477
TAGTATT86300.088.041226
TATTCCA97600.077.0083859
CATAGTA35700.068.6480944
TATAAAG79250.066.150665
TAAAGAC80300.065.834817
ATAACCG5800.062.1281051
AATAGTA50600.060.91644
ACGATCA8850.059.631367145
CTTTGAA63950.059.20162145
CGCACTA5050.057.561112
AAGACAC92250.057.3059779
GATAGTA20650.055.3634954
CGGACTA6000.054.766482
GCCCTCG30450.053.500916145
ATAATCG5250.051.7786141
AACTATT37300.051.1402784
AAGCTCG17000.049.7847949
Produced by FastQC (version 0.11.5)