FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5789106
Sequences flagged as poor quality0
Sequence length8-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT121800.096.511195
GTATTCC128700.091.082638
AGTATTC131200.090.7364047
TAGTATT132550.090.38496
CATAGTA62000.080.770294
TATTCCA150600.078.378469
TAAAGAC113850.065.828447
TATAAAG116900.064.49385
GTCTACT41000.064.202976
AAGCTGC7950.064.051871
AATAGTA64400.063.1252944
AAGACAC123850.060.207379
TATAGTA36900.053.6375774
TGTCTAC49800.053.239545
CGCGATA1800.052.3881071
TCGACAC7700.052.0334145
CTTTGAA85200.051.728043145
CCATAGT40300.051.6149443
AAGCTCG21650.051.06819
TGCTGCC3500.050.2812842
Produced by FastQC (version 0.11.5)