FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5839437
Sequences flagged as poor quality0
Sequence length20-137
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT349990.5993557255605292No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC118280.20255377359152943No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA86280.14775397011732466No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG85050.1456476026712849No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCATTATTCACAA59760.10233863298807744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC53700.0102.532141
GCATAGT120550.0100.916323
CTGCATA156000.098.079331
CTGCTGC53850.097.902731
GCTGCTA4900.097.890483
TGCTGCC15350.097.6513442
GCTGCAA9400.097.591093
CATAGTA116450.097.261074
ATAGTAT114500.096.036595
CTGCTAT144900.095.865391
CAGCAGC55200.095.073751
AGCTGCC16300.094.534892
TGCATAG130200.093.897252
GCTATAA102850.092.57483
TGCTATA107200.092.285992
CTGCAGC43450.092.210061
GTATTCC122350.089.18888
GCAGCAT7450.088.528483
AGTATTC126350.087.9312067
TAGTATT127800.086.605156
Produced by FastQC (version 0.11.5)