FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5839437
Sequences flagged as poor quality0
Sequence length20-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT126750.098.1708765
GTATTCC131400.093.780978
AGTATTC133950.093.167287
TAGTATT135450.092.6948556
CATAGTA61600.080.6231844
TATTCCA154900.078.984669
TCGACAC8200.070.35506145
ACGATCA10700.070.32647145
TATAAAG111600.066.2123955
TAAAGAC112350.066.044137
GATAGTA31700.064.96494
AATAGTA69550.062.3799634
GTCTACT38050.062.3651246
AAGACAC124700.058.9485059
TGCAGCC3500.055.6444742
TGTCTAC44550.053.4094965
CTTTGAA83800.052.38119145
AAGCTCG19500.051.1852539
TATTCCG8750.048.7846539
ATCCACA69800.047.794456145
Produced by FastQC (version 0.11.5)