FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3284723
Sequences flagged as poor quality0
Sequence length10-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT54150.093.031245
GTATTCC57650.086.607218
TAGTATT59650.085.620576
AGTATTC60300.084.378537
CGGACTA6950.080.292612
TATTCCA71000.070.67859
CGTACTA2850.068.975462
GATAGTA18750.065.906254
CCGTACT5250.065.2525561
CCGGACT13100.064.8928151
CGCACTA5200.064.632392
CATAGTA30100.063.581934
CGGGATA6300.063.4399871
CCGGATT7700.060.9682961
CCGGTAT4050.057.9575231
CCGTACA4650.055.9363331
CTTTGAA43450.055.465416145
CCGGTTT8400.055.1323661
CGAACTA3200.053.5046462
ACTATTC47200.053.163475
Produced by FastQC (version 0.11.5)