FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3363168
Sequences flagged as poor quality0
Sequence length19-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTAACACTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAA259020.770166699968601No Hit
AATTAACACTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACT49590.1474502611823138No Hit
AATTAACACTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGA47450.14108721300868704No Hit
AATTAACACTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCATT35400.10525789969457369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACAC3329800.0127.156883
AATTAAC3320850.0127.135771
ATTAACA3339950.0126.734152
TAACACT1653200.0126.264824
TAACACA1701500.0125.832284
AACACTG1668100.0125.1599665
ACACTGC1667600.0125.015146
ACACAGC1721750.0124.287346
AACACAG1725550.0124.1488655
CACAGCA661600.0115.87117
CACTGCG132950.0114.927887
CACTGCT597750.0113.965077
CACAGCG139550.0113.751187
CACTGCA694650.0112.584497
CACAGCT540650.0110.871727
CACAGCC562750.0110.84427
ACTGCAT205600.0110.241838
ACTGCTA135600.0109.989068
CACTGCC436900.0109.0142447
CTGCATA106500.0108.003469
Produced by FastQC (version 0.11.5)