FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3481735
Sequences flagged as poor quality0
Sequence length20-137
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT162840.4676978575336722No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC51200.1470531215040777No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA40030.11497141511344201No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG36860.10586675895781844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT60900.097.321123
CATAGTA57800.095.671394
CTGCATA80100.095.477541
GCTATAA50000.093.505933
ATAGTAT57700.093.231435
TGCATAG67000.092.052852
TGCTATA52300.091.924932
CTGCTAT76900.090.76531
AGTATTC64350.085.373457
TAGTATT65400.084.0027856
GTATTCC64000.083.9599468
CAGCTGC44800.082.7353061
CAGCAGC53100.079.548271
CTGCTGC43650.078.160441
AGCACTA44200.077.596252
CTGCAGC39150.075.6173551
CTATAAA52600.073.8994454
TATTCCA73400.073.290619
CTGCAGT113650.072.798331
CAGCACT110400.069.327611
Produced by FastQC (version 0.11.5)