FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5231683
Sequences flagged as poor quality0
Sequence length19-137
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT263060.5028209851399635No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG79520.15199697688105338No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC76450.14612888433798454No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA65120.1244723734217077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT106400.0100.511933
CTGCATA140750.098.0984041
CATAGTA102100.097.079364
ATAGTAT100550.095.012535
CTGCTAT124350.094.556121
TGCATAG116800.094.018442
GCTATAA79650.093.23773
CAGCTGC52750.092.0827941
CTGCTGC61250.091.3995061
TGCTATA85000.091.0251162
GTATTCC107450.087.910348
GCTGCTA6900.087.4537663
CTGCAGC51200.087.1687851
AGTATTC111350.085.9572757
TAGTATT113750.084.827316
CAGCAGC60800.081.561441
CTGCAGT172700.077.424451
AGCACTA61250.076.962872
TATTCCA125450.075.582429
TGCTGCC21350.074.158062
Produced by FastQC (version 0.11.5)